4/08/2005
.:. Work work Work... .:.
Work work Work...
wow! ang sarap tlga mg trabaho! my computer is fixed and i havnt been doing anything the whole afternoon that isnt internet related.lol.
oh well, as you can seen i hvnt written a post for two days na...coz sobrng bz ako.
The other day, one of the research assistants briefed me with the research protocols and methodologies that i will be using, namely fungi DNA extraction. Well, we also ran out of alot of buffers and reagents needed for the small experiment so, i found myself, running all over the lab, weighing, staring at graduated cyclinders and eventually washing every dang glassware i used. Nkkpagod in fairness (to whom?)...in fairness to the supposed simplicity of the task. By the end of the day, i was able to run my DNA extract through electrophoresis, determine the DNA concentration and adjust each sample to [20ug/uL].
yesterday, i planted 3 varieties of tomatoes for my research (w/c is btw: Race determination and IGS haplotype analysis of Fusarium Wilt Pathogens in Taiwan)...well, for each variety, there were 7 trays, each tray had 240 seeds that needed to be planted at equal distances to each other (12 columns X 20 rows). so mind you 240 seeds by hand in each tray. Buti nlng, there were 3 other workers or rather greenhouse assistants who helped me put peat moss into each tray, make small furrows, and plant as well. it was tiring, mind you.
By the time we finished, it was lunch time.
In the afternoon, i was free. but i decide to sleep instead of wasting myself in the computer room. lol.
Today, i was finally able to work on the 30 isolates of fusarium oxysporum f.sp. lycopersici (the causative agent of fusarium wilt in tomatoes of course). Sympre, lahat ng isolates came from stored spores in silica gel. So, firstly, first thing in the morning, i prepared the Potato Dextrose Agar (PDA), the media in w/c i shall propogate the isolates to work on. I think i made about 3L and prepared 30 plates for the fungal propagation. THe propagation, itself was easier than expected, just pour in the silica gel particles, maybe around 20 crystals or so, onto the PDA plates. By lunch, tapos na ako.
And i havnt been doing anything else except pretend that im researching in my room. lol.
haay..4pm na.
i posted a poll on the NAVS yGroup...\lol...first time ko mggnyn..
sna mdmi ssgot dun.
thats all for now.
losing my grip. [1:06 PM]
_____________
